The efflux pump SmeDEF contributes to trimethoprim / sulfamethoxazole 1 resistance in
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چکیده
The efflux pump SmeDEF contributes to trimethoprim/sulfamethoxazole 1 resistance in Stenotrophomonas maltophilia 2 3 María Blanca Sánchez and José Luis Martínez* 4 Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, 5 Darwin 3, Cantoblanco, 28049 Madrid, Spain. 6 7 Corresponding author’s mailing address: 8 JLM e-mail: [email protected] 9 10 11 12 13 14 15 16 17 18 19 20 21 AAC Accepted Manuscript Posted Online 27 April 2015 Antimicrob. Agents Chemother. doi:10.1128/AAC.00714-15 Copyright © 2015, American Society for Microbiology. All Rights Reserved.
منابع مشابه
The efflux pump SmeDEF contributes to trimethoprim-sulfamethoxazole resistance in Stenotrophomonas maltophilia.
Trimethoprim-sulfamethoxazole (co-trimoxazole) is one of the antimicrobials of choice for the treatment of Stenotrophomonas maltophilia infections. The analysis of mutants either lacking or overexpressing the efflux pump SmeDEF shows that this efflux pump contributes to intrinsic and acquired co-trimoxazole resistance in S. maltophilia. Since SmeDEF can extrude a variety of antibiotics, selecti...
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OBJECTIVES The contribution of integrons and efflux pumps to multidrug resistance in Stenotrophomonas maltophilia was evaluated. MATERIALS AND METHODS Ninety-three S. maltophilia clinical isolates were studied. PCR and direct sequencing were used to detect the presence of integrons. Real-time PCR was performed to assess and quantify the expression of the Sme efflux pumps of S. maltophilia. ...
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متن کاملA function of SmeDEF, the major quinolone resistance determinant of Stenotrophomonas maltophilia, is the colonization of plant roots.
Quinolones are synthetic antibiotics, and the main cause of resistance to these antimicrobials is mutation of the genes encoding their targets. However, in contrast to the case for other organisms, such mutations have not been found in quinolone-resistant Stenotrophomonas maltophilia isolates, in which overproduction of the SmeDEF efflux pump is a major cause of quinolone resistance. SmeDEF is ...
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OBJECTIVES The use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. However, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. To test the effect of antibiotic resistance on...
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